Using Methylcellulose during Culturing and Cryopreservation of SPEV Passaged Culture

Authors

  • S. V. Koshchiy Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • I. P. Vysekantsev Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • V. F. Martsenyuk Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • T. F. Petrenko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • E. V. Kudokotseva Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Keywords:

cryopreservation, culturing, methylcellulose, passaged culture, serum

Abstract

The possibility of adaptation of cultured embryonic pig kidney cells (SPEV) to growth in culturing medium 199+0.1% MC with reduced serum content to 1% has been shown. Cryoprotective properties of MC for the cryopreservation of SPEV have been determined.

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Published

2008-11-20

How to Cite

Koshchiy, S. V., Vysekantsev, I. P., Martsenyuk, V. F., Petrenko, T. F., & Kudokotseva, E. V. (2008). Using Methylcellulose during Culturing and Cryopreservation of SPEV Passaged Culture. Problems of Cryobiology and Cryomedicine, 18(3), 285–288. Retrieved from https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/390

Issue

Vol. 18 No. 3 (2008): Problems of Cryobiology

Section

Conference Papers

License

Copyright (c) 2020 S. V. Koshchiy, I. P. Vysekantsev, V. F. Martsenyuk, T. F. Petrenko, E. V. Kudokotseva

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