Character of Changes of Structural and Functional Parameters of Fetal Liver Cells after Cryopreservation Depending on Their Gestation Terms

Authors

  • M. V. Ostankov Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • N. A. Bondarovich Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • I. V. Rassokha Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • A. N. Goltsev Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Keywords:

fetal liver cells, hemopoietic stem cells, C3H mice, breast cancer

Abstract

The efficiencies of three freezing programs (two, three- and four-stage) basing on the estimation of morphofunctional state and therapeutic potential of cryopreserved fetal liver cells (СFLCs) of 15 and 19 gestation days were studied. The investigations have shown that during freezing of FLC-15 the most effective was two-stage cryopreservation regimen under 10% DMSO protection, which provided higher content of survived and hemopoietic stem cells (CD34+CD38–). From this point of view the most suitable for cFLC-19 was the use of 12.5% DMSO at three-stage freezing program. In C3H mice genetically determined to the development of breast cancer the most manifested reduction of the frequency of tumor development and higher survival were observed after application of cFLC-15.

Downloads

Published

2008-11-20

How to Cite

Ostankov, M. V., Bondarovich, N. A., Rassokha, I. V., & Goltsev, A. N. (2008). Character of Changes of Structural and Functional Parameters of Fetal Liver Cells after Cryopreservation Depending on Their Gestation Terms. Problems of Cryobiology and Cryomedicine, 18(3), 302–305. Retrieved from https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/395

Issue

Vol. 18 No. 3 (2008): Problems of Cryobiology

Section

Conference Papers

License

Copyright (c) 2020 M. V. Ostankov, N. A. Bondarovich, I. V. Rassokha, A. N. Goltsev

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Authors who publish with this journal agree to the following terms:

  • Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  • Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  • Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).