Comparative Study of Preliminarily Dehydrated Human and Bovine Erythrocyte Sensitivity to Hypertonic Stress

Authors

  • D. I. Aleksandrova Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • N. V. Orlova Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • N. M. Shpakova Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Keywords:

human and bovine erythrocytes, hyperosmotic stress, preliminary cell dehydration, hematocrit

Abstract

Preliminary incubation of human and bovine erythrocytes in 0.4 and 1.0 M NaCl, correspondingly, results in the formation of a resistant cell state to the following 4.0 M NaCl effect. Maximum erythrocyte shrinking at the stage of preliminary incubation in moderately hypertonic media was shown as correlating with the minimum hemolysis level under cell transfer into 4.0 M NaCl-containing medium. At 37°C the level of minimum hypertonic hemolysis and minimum hematocrit value for bovine erythrocytes is lower that the similar ones, obtained for human erythrocytes. The reduction of experimental temperature down to 0°C results in hemolysis level and human erythrocyte volume decrease compared to 37°C.

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Published

2007-12-15

How to Cite

Aleksandrova, D. I., Orlova, N. V., & Shpakova, N. M. (2007). Comparative Study of Preliminarily Dehydrated Human and Bovine Erythrocyte Sensitivity to Hypertonic Stress. Problems of Cryobiology and Cryomedicine, 17(4), 327–334. Retrieved from https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/479

Issue

Vol. 17 No. 4 (2007): Problems of Cryobiology

Section

Theoretical and Experimental Cryobiology

License

Copyright (c) 2020 D. I. Aleksandrova, N. V. Orlova, N. M. Shpakova

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