Mechanism of Post-hypertonic Lysis of Isolated Rat Hepatocytes During Rehydration

Authors

  • L. G. Kuleshova Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Keywords:

light microscopy, rat hepatocytes, hypertony, rehydration, membrane, post-hypertonic lysis

Abstract

The kinetics of cell volume control in the isolated rat hepatocytes when varying an extracellular medium tonicity, simulating the processes of dehydration and rehydration of cells under the conditions of growth and melting of extracellular ice crystals was studied with light microscopy. Post-hypertonic lysis of rat hepatocytes was shown to be realised by either discrete release of a part of endocellular content, or its continuous outflow to extracellular environment. Cell osmotic activity under following cyclic osmotic loadings testified to a preservation of membrane semi-permeable properties and “healing†of the membrane pore.

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Published

2006-12-15

How to Cite

Kuleshova, L. G. (2006). Mechanism of Post-hypertonic Lysis of Isolated Rat Hepatocytes During Rehydration. Problems of Cryobiology and Cryomedicine, 16(4), 351–362. Retrieved from https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/569

Issue

Vol. 16 No. 4 (2006): Problems of Cryobiology

Section

Theoretical and Experimental Cryobiology

License

Copyright (c) 2020 L. G. Kuleshova

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