Premises for Optimizing the Method of Bone Marrow Cryopreservation in Animals with Autoimmune Pathologies

Authors

  • Yu. A. Kozlova Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • A. N. Goltsev Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • T. G. Dubrava Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • T. M. Gurina Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Keywords:

bone marrow, autoimmune diseases, CFU-GM

Abstract

There were determined the functional characteristics of bone marrow cells (BM) in healthy animals and donors with autoimmune diseases (AID) prior to and following cryopreservation. The studies demonstrated that independently on the type of autoimmune pathology, the maximum amount of hemopoietic precursors of granulomonocytopoesis (CFU-GM) in BM was reached during its cryopreservation under the protection of 7% DMSO. For BM cells of intact animals the same integrity of CFU-GM was obtained when using DMSO in 10% concentration.

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Published

2004-06-15

How to Cite

Kozlova, Y. A., Goltsev, A. N., Dubrava, T. G., & Gurina, T. M. (2004). Premises for Optimizing the Method of Bone Marrow Cryopreservation in Animals with Autoimmune Pathologies. Problems of Cryobiology and Cryomedicine, (2), 76–83. Retrieved from https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/692

Issue

No. 2 (2004): Problems of Cryobiology

Section

Theoretical and Experimental Cryobiology

License

Copyright (c) 2020 Yu. A. Kozlova, A. N. Goltsev, T. G. Dubrava, T. M. Gurina

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