Features of Protein-Peptide and Carbohydrate Composition of Supernatants From Tenebrio Molitor Larvae After Cold Acclimation

Authors

  • Aleksandr K. Gulevsky Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv
  • Diana V. Tretiak Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

DOI:

https://doi.org/10.15407/cryo26.04.322

Keywords:

Tenebrio molitor, cold acclimation, chromatography, peptides, sugars, sugar alcohols

Abstract

The paper describes the chromatographic studies on the molecular-mass distribution of proteins and peptides of supernatants, derived from Tenebrio molitor larvae during cold acclimation. Supernatants of non-acclimated larvae of T. molitor had the highest amount of peptide fractions. It has been shown that cold-acclimated T. molitor larvae comprised the low-molecular peptide fractions with MW of (540 ± 20) – (2.255 ± 85) Da, and high-molecular peptides with MW of (4.675 ± 225) – (6.595 ± 550) Da were characteristic for non-acclimated larvae. The content of sugars and polyols in supernatants of cold-acclimated and non-acclimated T. molitor larvae was determined. It has been found that glucose concentration in non-acclimated T. molitor larvae was in 1.34 times higher than in cold-acclimated ones. Ðcclimated T. molitor larvae had more hydrophilic and less hydrophobic proteins as compared to non-acclimated ones. As well it has been established that non-acclimated T. molitor larvae had low concentrations of polyols (sorbitol and mannite), which were absent in acclimated insects.

Probl Cryobiol Cryomed 2016; 26(4): 322–330

Author Biographies

Aleksandr K. Gulevsky, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cold Adaptation

Diana V. Tretiak, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cold Adaptation

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Published

2016-12-23

How to Cite

Gulevsky, A. K., & Tretiak, D. V. (2016). Features of Protein-Peptide and Carbohydrate Composition of Supernatants From Tenebrio Molitor Larvae After Cold Acclimation. Problems of Cryobiology and Cryomedicine, 26(4), 322–330. https://doi.org/10.15407/cryo26.04.322

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Section

Theoretical and Experimental Cryobiology