Study of Morphological Characteristics of Cryopreserved Cell Cultures of Stromal Origin

Authors

  • N O. Volkova Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Keywords:

cryopreservation, cell culture, proliferation, differentiation

Abstract

Mesenchymal stromal cells (MSCs) of bone marrow and chorion cell culture (CCC) were cryopreserved under protection of 10% DMSO and 20% fetal bovine serum using low cooling rate (1 deg/min) down to –80°C with following plunging into liquid nitrogen. The findings have shown than after low temperature storage both studied cultures kept the ability to proliferation and directed differentiation. Chorion cell culture was characterized with higher proliferative activity if compared with bone marrow MSCs. The ability to the directed differentiation towards adipo- and chondrogeneic lineages was higher for bone marrow MSCs versus CCC.

Downloads

Published

2012-06-18

How to Cite

Volkova, N. O. (2012). Study of Morphological Characteristics of Cryopreserved Cell Cultures of Stromal Origin. Problems of Cryobiology and Cryomedicine, 22(2), 118–125. Retrieved from https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/72

Issue

Vol. 22 No. 2 (2012): Problems of Cryobiology

Section

Theoretical and Experimental Cryobiology

License

Copyright (c) 2020 N O. Volkova

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Authors who publish with this journal agree to the following terms:

  • Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  • Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  • Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).