Dynamics of Morphological Change in Neonatal Ovarian Tissue During Hypothermic Storage Depending on Composition of Incubation Medium

Authors

  • V. V. Kiroshka Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • E. A. Medinets Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • Yu. O. Tischenko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • T. P. Bondarenko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Keywords:

hypothermic storage, morphological structure, neonatal ovarian tissue, electrolyte, nonelectrolyte

Abstract

In this work we carried-out the comparative analysis of dynamics of morphological structure transformation of neonatal ovarian tissue during hypothermic storage (HS) in the media with different composition. It has been found that high pool of morphologically normal follicles (88%) was preserved in nonelectrolyte solutions, formation of degenerative follicles was of reversible character. In electrolyte solutions the quantity of morphologically normal follicles was lower (66%), the damages were accompanied by the formation of irreversible degenerative forms of follicles. It has been demonstrated experimentally that the neonatal ovarian tissue after the one day of HS in nonelectrolyte solutions had the ability to restore its function post heterotopic transplantation.

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Published

2012-03-14

How to Cite

Kiroshka, V. V., Medinets, E. A., Tischenko, Y. O., & Bondarenko, T. P. (2012). Dynamics of Morphological Change in Neonatal Ovarian Tissue During Hypothermic Storage Depending on Composition of Incubation Medium. Problems of Cryobiology and Cryomedicine, 22(1), 61–70. Retrieved from https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/98

Issue

Vol. 22 No. 1 (2012): Problems of Cryobiology

Section

Theoretical and Experimental Cryobiology

License

Copyright (c) 2020 V. V. Kiroshka, E. A. Medinets, Yu. O. Tischenko, T. P. Bondarenko

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