Effect of Combined Media on Damage of Erythrocytes, Frozen with Different Hematocrit Values

Authors

  • V. V. Ramazanov Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Keywords:

erythrocytes, combined cryopreservatives, “packing” effect

Abstract

Damage of erythrocytes, frozen with low (0.8%) and high (40%) hematocrit values in media with non-penetrative (polyethylene glycol with molecular mass of 1500 and 2000 (PEG-1500, PEG-2000), dextrans) and penetrative (glycerol, dimethylsufoxide, 1,2-propane diol, PEG-400, glucose) cryoprotectants has been studied. Freezing of erythrocytes with high hematocrit in sucrose-saline medium was demonstrated as resulting in higher cell damages if to compare with a low hematocrit. Combining this mentioned medium with non-penetrative cryoprotectant does not eliminate the “packing†effect, while the penetrative ones realise it. The results obtained indicate to the fact, that in order to eliminate the damage, relating to high cell density in freezing medium the combination of sucrose with penetrative cryoprotectants is needed.

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Published

2006-06-15

How to Cite

Ramazanov, V. V. (2006). Effect of Combined Media on Damage of Erythrocytes, Frozen with Different Hematocrit Values. Problems of Cryobiology and Cryomedicine, 16(2), 155–163. Retrieved from https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/596

Issue

Vol. 16 No. 2 (2006): Problems of Cryobiology

Section

Theoretical and Experimental Cryobiology

License

Copyright (c) 2020 V. V. Ramazanov

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