Structure of Myocardium of Young Hypertensive Rats after Injection of Cryopreserved Human Cord Blood Nucleated Cells

Authors

  • Lyudmila V. Babijchuk Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv
  • Sergey N. Koval L.T. Malaya National Institute of Therapy of the National Academy of Medical Sciences of Ukraine, Kharkiv
  • Georgiy A. Babijchuk Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

DOI:

https://doi.org/10.15407/cryo26.03.271

Keywords:

arterial hypertension, cryopreserved cord blood nucleated cells, cardiomyocytes, myocardial ischemia, cardiosclerosis, fibrosis

Abstract

Nowadays cell therapy is considered as a promising new method to treat various diseases of cardiovascular system. Experimental model of stress-induced arterial hypertension (AH) was used to study the histological features of heart tissues and blood vessels: we have performed histochemical and polarization-optical assessment of collagen and elastic fibers of myocardium of young rats before and after introduction of human cryopreserved cord blood nucleated cells (cCBNCs). It has been found that myocardium structure of young rats to day 30 after the hypertension onset had pathological changes. We have found the changes in muscle fibers, spasm of blood vessels, paresis of the veins and capillaries, perivascular edema and cardiosclerosis. To day 3 after application of cCBNCs the number and area of cardiomyocyte nuclei increased. These compensatory changes were accompanied with a pronounced neoangiogenesis. One month later the administration of cCBNCs  the density of fibroblasts in cardiac muscle of hypertensive rats increased (number of fibroblasts per area unit) in interstitial connective tissue and around the blood vessels; no areas with signs of focal ischemia and cardiosclerosis were noted. Perivascular and interstitial edemas were less pronounced.

 

Probl  Cryobiol Cryomed 2016; 26(3): 271–287

Author Biographies

Lyudmila V. Babijchuk, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cryophysiology

Georgiy A. Babijchuk, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cryophysiology

References

Babiychuk L.A., Grishchenko V.I., Ryazantsev V.V. et al., inventors. The method of isolation of nucleated cord blood cells. Patent of Ukraine Nr. 234999, IPC C 12 N 5/00, 2007 May. 25.

Babiychuk L.A., Grishchenko V.I., Gurina T.N. et al., inventors. The method of cryopreservation of cord blood yadrovmisnyh cells, including hematopoietic stem cells. Patent of Ukraine Nr. 92227, IPC A 01 N 1/02, 2010 Oct. 11.

Babiychuk L.V., Babiychuk V.H., Kozlov A.V. et al., inventors. Method of simulation of stress-induced hypertension. Patent of Ukraine Nr. 67199, IPC G 09 B 23/28, 2012 Jan. 10.

Belaya V.N. Myocardium remodeling mechanisms in arterial hypertension. International Medical Journal 2006; 12(2): 15–18.

Belenkov Yu.N., Privalov E.V., Chekneva I.S. Cell therapy of chronic heart failure: types of stem cells used the results of recent clinical studies. Cardiovascular Surgery 2008; 5: 4–18.

Berk C., Fujjwara S., Lehoux S. ECM remodeling in hypertensive heart disease. J Clin Invest 2007; 117(3): 568–575. CrossRef PubMed

Eirin A., Zhu X.Y., Ferguson C.M. et al. Intra-renal delivery of mesenchymal stem cells attenuates myocardial injury after reversal of hypertension in porcine renovascular disease. Stem Cell Res Ther 2015; 6(1): 7. CrossRef PubMed

Gavriliuk V.A. Structural functional remodeling of the heart and vessels in patients with essential arterial hypertension with different body mass. International Medical Journal 2012; 18(1): 38–41.

Kochegura T.N., Efimenko A.Y., Akopyan Zh. A. et al. Stem cell therapy of heart failure: clinical trials, problems and per-spectives. Cellular Transplantation and Tissue Engineering 2010; 5(2): 11–18.

Kovalenko V.N. Guide to Cardiology. Kyiv: Morion, 2008.

Kovalenko V.M., Sirenko Y.M., Radchenko G.D. The stress and the occurrence of hypertension: what is known. Arteryalnaya Hypertensiya 2014; 36(4): 9–20.

Kondakov I.I. Anti-atherogenic effects of the drug cryopreserved fetoplacental complex in experimental atheros-clerosis. Problems of Cryobiology 2005; 15(3): 435–439.

Kulovskyy V.I., Serouhova A.P. Microcirculation disorders in patients arteryalnoy hypertension. Vestnik Vitebskogo Gosudarstvennoho Meditsinskogo Universiteta 2008; 7(11): 1–11.

Kopica N.P., Bella N.B., Titarenko N.V. Methods of diagnosis of fibrosis in patients with myokardyal arterial hypertension. Arteryalnaya Hypertensiya 2008; 2(2): 10–15.

Nasadyuk H.M. Cord blood stem cells in regenerative therapy of diseases of the cardiovascular system. Zhinochiy Likar 2009; 6: 12–15.

Sarkisov D.S., Petrov D.S. Microscopical technics. Moscow: Meditsina, 1996.

Sirenko Y.N. Diagnosis, prevention and treatment of arterial hypertension. Meditsina Ukrainy 2004; 79(3): 6–9.

Strukov A.I., Serov V.V. Patological anatomy, Moscow: Meditsina, 1993. PubMed

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Published

2016-09-23

How to Cite

Babijchuk, L. V., Koval, S. N., & Babijchuk, G. A. (2016). Structure of Myocardium of Young Hypertensive Rats after Injection of Cryopreserved Human Cord Blood Nucleated Cells. Problems of Cryobiology and Cryomedicine, 26(3), 271–287. https://doi.org/10.15407/cryo26.03.271

Issue

Section

Cryomedicine, Clinical and Experimental Transplantology