Features of Protein-Peptide and Carbohydrate Composition of Supernatants From Tenebrio Molitor Larvae After Cold Acclimation
Keywords:Tenebrio molitor, cold acclimation, chromatography, peptides, sugars, sugar alcohols
The paper describes the chromatographic studies on the molecular-mass distribution of proteins and peptides of supernatants, derived from Tenebrio molitor larvae during cold acclimation. Supernatants of non-acclimated larvae of T. molitor had the highest amount of peptide fractions. It has been shown that cold-acclimated T. molitor larvae comprised the low-molecular peptide fractions with MW of (540 Â± 20) â€“ (2.255 Â± 85) Da, and high-molecular peptides with MW of (4.675 Â± 225) â€“ (6.595 Â± 550) Da were characteristic for non-acclimated larvae. The content of sugars and polyols in supernatants of cold-acclimated and non-acclimated T. molitor larvae was determined. It has been found that glucose concentration in non-acclimated T. molitor larvae was in 1.34 times higher than in cold-acclimated ones. Ðcclimated T. molitor larvae had more hydrophilic and less hydrophobic proteins as compared to non-acclimated ones. As well it has been established that non-acclimated T. molitor larvae had low concentrations of polyols (sorbitol and mannite), which were absent in acclimated insects.
Probl Cryobiol Cryomed 2016; 26(4): 322â€“330
Bennett V.A., Sformo T., Walters K. et al. Comparative overwintering physiology of Alaska and Indiana populations of the beetle Cucujus clavipes (Fabricius): roles of antifreeze proteins, polyols, dehydration and diapauses. J Exp Biol 2005; 208(23): 4467â€“4477. CrossRef PubMed
Bidlingmayer B. Preparative liquid chromatography. Moscow: Mir, 1990.
Celik Y., Drori R., Pertaya-Braun N. et al. Microfluidic experiments reveal that antifreeze proteins bound to ice crystals suffice to prevent their growth. PNAS 2013; 110(4): 1309â€“1314. CrossRef PubMed
Davies P.L., Hew C.L. Biochemistry of fish antifreeze proteins. The FASEB J. 1990; 4(8): 2460â€“2468. PubMed
Duman J.G. Environmental effects on antifreeze levels in larvae of the darkling beetle, Meracantha contracta. J Exp Zool 1977; 201(2): 333â€“337. CrossRef
Duman J.G. Factors involved in overwintering survival of the freeze tolerant beetle, Dendroides Canadensis. J Comp Physiol 1980; 136(1): 52â€“59. CrossRef
Franks F. Protein destabilization at low temperatures. Adv Prot Chem 1995; 46: 105â€“139. CrossRef
Garnham C.P., Natarajan A., Middleton A.J. et al. Compound icebinding site of an antifreeze protein revealed by mutagenesis and fluorescent tagging. Biochemistry 2010; 49(42): 9063â€“9071. CrossRef PubMed
Graham L.A., Walker V.K., Davies P.L. Developmental and environmental regulation of antifreeze proteins in the mealworm beetle Tenebrio molitor. Eur J Biochem 2000; 267(21): 6452â€“6458. CrossRef PubMed
Gulevsky A.K., Ryazantsev V.V., Grischenkova Ye.A., Relina L.I. Changes in protein composition of mealworms (Tenebrio molitor) during cold acclimation. Problems of Cryobiology 1995; (4): 29â€“32.
Jaenicke R., Bohm G. The stability of proteins in extreme environments. Curr Opin Struct Biol 1998; 8(6): 738â€“748. CrossRef
Kahlke T., Thorvaldsen S. Molecular characterization of cold adaptation of membrane proteins in the Vibrionaceae coregenome. PLOS One 2012; 7(12): e51761. CrossRef
Liou Y.C., Thibault P., Walker V.K. et al. A complex family of highly heterogeneous and internally repetitive hyperactive antifreeze proteins from the beetle Tenebrio molitor. Biochemistry 1999; 38(35): 11415â€“11424. CrossRef PubMed
Patterson J.L., Duman J.G. Purification and composition of protein antifreezes with high cysteine contents from larvae of the beetle, Tenebrio molitor. J Exp Zool 1982; 219(3): 381â€“384. CrossRef
Posner M. Functional validation of hydrophobic adaptation to physiological temperature in the small heat shock protein A-crystallin. PLOS One 2012; 7(3): e34438. CrossRef
Scopes R. Methods of protein purification. Moscow: Mir; 1985. 20.Singh P., Hanada Y., Singh S.M. et al. Antifreeze protein activity in Arctic cryoconite bacteria. FEMS Microbiol Lett 2014; 351(1): 14â€“22. CrossRef
Wohrmann A.P.A. Antifreeze glycopeptides and peptides in Antarctic fish species from the Weddell Sea and the Lazarev Sea. Mar Ecol Prog Ser 1996; 130: 47â€“59. CrossRef
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