Effect of Freezing Regimens on Protein Aggregation of Human Cord Blood Serum

Authors

  • E. O. Nardid Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • E. I. Naumenko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • S. L. Rozanova Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • O. A. Nardid Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Keywords:

cord blood, serum, aggregation of blood components, freezing regimens, gel chromatography

Abstract

The effect of different freezing regimens on protein spectrum of cord blood serum has been studied. It has been established that the most favourable conditions are freezing of cord blood serum with the rate not less than 100ºC/min and storage temperature of –80ºC and lower. Cooling rates and storage temperature realized when placing the samples into cooling chambers do not enable the prevention of low temperature aggregation of proteins.

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Published

2006-09-15

How to Cite

Nardid, E. O., Naumenko, E. I., Rozanova, S. L., & Nardid, O. A. (2006). Effect of Freezing Regimens on Protein Aggregation of Human Cord Blood Serum. Problems of Cryobiology and Cryomedicine, 16(3), 311–315. Retrieved from http://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/586

Issue

Section

Cryopreservation of Biological Resources