Effect of Cryopreservation Factors on the Energetic Metabolism of Cyprinus carpio L. activated Spermatozoa

Authors

  • V. V. Cherepanov

Keywords:

cryopreservation, fish sperm, creatinephosphate, ATP, lactate, pyruvate, glucose-6-phosphate

Abstract

We have investigated the cryopreservation factors (hypothermia, hypertonia, freezing and thawing) on the content of such key energetic metabolites as glucose-6-phosphate (G6P), pyruvate (PV), lactate and main macroergic phosphates of ATP and creatinephosphate (CP) in Cyprinus carpioL. spermatozoa to reveal the most cryosensitive metabolic reactions. It has been established that all the factors studied cause significant alterations in an energy metabolism of the majority of cells; the methods for fish sperm cryopreservation used do not provide a complete integrity of internal functional parameters for all the cells.

Author Biography

V. V. Cherepanov

Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

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Published

2003-12-25

How to Cite

Cherepanov, V. V. (2003). Effect of Cryopreservation Factors on the Energetic Metabolism of Cyprinus carpio L. activated Spermatozoa. Problems of Cryobiology and Cryomedicine, (4), 58–65. Retrieved from http://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/1079

Issue

No. 4 (2003): Problems of Cryobiology

Section

Theoretical and Experimental Cryobiology

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