Changes in Ovarian Tissue Structure Following Saturation with 1,2-Propanediol in Media of Various Compositions

Authors

  • Irina A. Trutayeva Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • Viktoriya V. Kiroshka Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • Yuliya O. Bozhkova Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • Tatyana P. Bondarenko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

DOI:

https://doi.org/10.15407/cryo26.01.053

Keywords:

ovarian tissue, 1, 2-propane diol, oocyte, degenerative follicle

Abstract

One of the most promising technologies of preserving fertility in women with ovarian pathologies of various origins is the transplantation of cryopreserved ovarian tissue. Wide introduciton of this technology into a clinical practice requires the optimizing of freeze-thawing protocols. One of the stages of improving the cryopreservation process is the enhancing of osmotic tolerance of cells in the tissue when adding the cryoprotective solutions. In this research there was comparatively analyzed the dynamics of volu-metric and morphological transformation of ovarian tissue at its gradual saturation with the 1,2-PD (1.5–3 M) in the media of different composition. The preservation of follicle structure integrity in the final concentration of 1,2-PD (3 M) was found to be dependent on quantitative ratios of electrolyte and non-electrolyte components of the initial medium. An increase in the initial medium osmolarity by introducing a non-electrolyte component (sucrose) resulted in a decrease in the volume of oocytes and maintaining of cell-to-cell contacts of the follicle structural components.


Probl Cryobiol Cryomed 2016; 26(1):53-62.

Author Biographies

Irina A. Trutayeva, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Department of Biochemistry and Pharmacology of Neurohumoral Systems

Viktoriya V. Kiroshka, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Department of Biochemistry and Pharmacology of Neurohumoral Systems

Yuliya O. Bozhkova, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Department of Biochemistry and Pharmacology of Neurohumoral Systems

Tatyana P. Bondarenko, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Department of Biochemistry and Pharmacology of Neurohumoral Systems

References

Amorim C.A., Rondina D., Rodrigues A.P.R. et al. Cryopreservation of isolated ovine primordial follicles with propylene glycol and glycerol. Fertil Steril 2004; 81(1): 735–740. CrossRef PubMed

Andersen C.Y., Rosendahl M., Byskov A.G. et al. Two successful pregnancies following autotransplantation of frozen/thawed ovarian tissue. Hum Reprod 2008; 23 (10): 2266–2272. CrossRef PubMed

Banderali U., Roy G. Activation of K+ and Cl– channels in MDCK cells during volume regulation in hypotonic media. J Membrane Biol 1992; 126: 219–234. CrossRef PubMed

Bortner C.D., Hughes F.M., Cidlowski J.Ð. A primary role for K+ and Na+ efflux in the activation of apoptosis. J Biol Chem 1997; 272 (51): 32436–32442. CrossRef PubMed

Comizzoli P., Wildt D.E., Pukazhenthi B.S. Impact of anisosmotic conditions on structural and functional integrity of cumulus-oocyte complexes at the germinal vesicle stage in the domestic cat. Mol Reprod Dev 2008; 75(2): 345–354. CrossRef PubMed

Demeestere I., Simon P., Buxant F. et al. Ovarian function and spontaneous pregnancy after combined heterotopic and orthotopic cryopreserved ovarian tissue transplantation in a patient previously treated with bone marrow transplantation: case report. Hum Reprod 2006; 21: 2010–2014. CrossRef PubMed

Demeestere I., Simon P., Emiliani S. et al. Options to preserve fertility before oncological treatment: cryopreservation of ovarian tissue and its clinical application. Acta Clin Belg 2006; 61(5): 259–263. CrossRef PubMed

Demeestere I., Simon P., Emiliani S. et al. Orthotopic and hetero-topic ovarian tissue transplantation. Hum Reprod Update 2009; 15(6): 649–665. CrossRef PubMed

Eyden B., Radford J., Shalet S.M. et al. Ultrastructural preservation of ovarian cortical tissue cryopreserved in dimethylsulfoxide for subsequent transplantation into young female cancer patients. Ultrastruct Pathol 2004; 28: 239–245. CrossRef PubMed

Gougeon A. Dynamics of follicular growth in the human: a model from preliminary results. Hum Reprod 1986; 1(2): 81–87. PubMed

Haussinger D. The role of cellular hydration in the regulation of cell function. Biochem J 1996; 313: 697–710. CrossRef PubMed

Lang F. Mechanisms and significance of cell volume regulation. J Am Coll Nutr 2007; 26(5): 613–623. CrossRef

Liu J., Mullen S., Meng Q. et al. Determination of oocyte membrane permeability coefficients and their application to cryopreservation in a rabbit model. Cryobiology 2009; 59(2): 127–134. CrossRef PubMed

Le Gal F., Gasqui P., Renard J.P. Differential osmotic behavior of mammalian oocytes before and after maturation: a quan-titative analysis using goat oocytes as a model. Cryobiology 1994; 31: 154–170. CrossRef PubMed

Newton H., Pegg D.E., Barrass R., Gosden R.G. Osmotically inactive volume, hydraulic conductivity, and permeability to dimethyl sulphoxide of human mature oocytes. J Reprod Fertil 1999; 117: 27–33. CrossRef PubMed

Okada Y., Maeno E., Shimizu T. et al. Receptor-mediated control of regulatory volume decrease (RVD) and apoptotic volume decrease (AVD). J Physiol 2001; 532(1): 3–16. CrossRef PubMed

Paynter S.J., Cooper A., Fuller B.J., Shaw R.W. Cryopreservation of bovine ovarian tissue: structural normality of follicles after thawing and culture in vitro. Cryobiology 1999; 38: 301–309. CrossRef PubMed

Paynter S. J., Fuller B. J. Shaw R. W. Temperature dependence of Kedem-Katchalsky membrane transport coefficients for mature mouse oocytes in the presence of ethylene glycol. Cryobiology 1999; 39: 169–176. CrossRef PubMed

Santos R.R., Hurk R., Rodrigues A.P.R. et al. Effect of cryopreservation on viability, activation and growth of in situ and isolated ovine early-stage follicles. Animal Reprod Sci 2007; 99: 53–64. CrossRef PubMed

Songsasen N., Ratterree M.S., VandeVoort C.A. et al. Permeability characteristics and osmotic sensitivity of Rhesus monkey (Macaca mulatta) oocytes. Hum Reprod 2002; 17(7): 1875–1884. CrossRef PubMed

Wang L., Liu J., Zhou G.-B. et al. Quantitative investigations on the effects of exposure durations to the combined cryoprotective agents on mouse oocyte vitrification procedures. Biol Reprod 2011; 85: 884–894. CrossRef PubMed

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Published

2016-03-21

How to Cite

Trutayeva, I. A., Kiroshka, V. V., Bozhkova, Y. O., & Bondarenko, T. P. (2016). Changes in Ovarian Tissue Structure Following Saturation with 1,2-Propanediol in Media of Various Compositions. Problems of Cryobiology and Cryomedicine, 26(1), 53–62. https://doi.org/10.15407/cryo26.01.053

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Section

Theoretical and Experimental Cryobiology