Antihemolytic Efficiency of Chlorpromazine under Posthypertonic Shock and Glycerol Removal from Erythrocytes after Thawing

Authors

  • Yekaterina A. Semionova Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv
  • Nina G. Zemlyanskikh Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv
  • Nataliya V. Orlova Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv
  • Nataliya M. Shpakova Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

DOI:

https://doi.org/10.15407/cryo27.01.051

Keywords:

erythrocytes, human, posthypertonic shock, cryopreservation, cryoprotectant removal, glycerol, chlorpromazine

Abstract

The chlorpromazine effect on sensitivity of human erythrocytes to the action of posthypertonic shock and their resistance at glycerol removal from the cells exposed to freeze-thawing has been studied in our research. It has been shown that under conditions of posthypertonic shock of erythrocytes (at 0°C) the chlorpromazine reduced a hemolysis level in 3.7 times, and in case of 600 µmol/l concentration its maximum antihemolytic activity was 73 ± 6%. The application of chlorpromazine during removal of glycerol from frozen-thawed erythrocytes enabled to reduce the cell damage by 2.5–3 times. The values of antihemolytic activity of chlorpromazine in both cases were quite similar. Results of the investigation of chlorpromazine efficiency in the model experiment and under the real conditions of erythrocyte freeze-thawing (under glycerol removal from the cells) allow to conclude about the applicability of this model (posthypertonic shock) in the further studies.

Probl Cryobiol Cryomed 2017; 27(1): 51–60

Author Biographies

Yekaterina A. Semionova, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cryocytology

 

Nina G. Zemlyanskikh, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cryocytology

 

Nataliya V. Orlova, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cryocytology

Nataliya M. Shpakova, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cryocytology

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Published

2017-03-25

How to Cite

Semionova, Y. A., Zemlyanskikh, N. G., Orlova, N. V., & Shpakova, N. M. (2017). Antihemolytic Efficiency of Chlorpromazine under Posthypertonic Shock and Glycerol Removal from Erythrocytes after Thawing. Problems of Cryobiology and Cryomedicine, 27(1), 51–60. https://doi.org/10.15407/cryo27.01.051

Issue

Section

Theoretical and Experimental Cryobiology