Effect of Sugars on Dehydration of Biopolymer Molecules During Cryopreservation

Authors

  • A. A. Rugal Institute of Surface Chemistry named by A.A. Chuyko of the National Academy of Sciences of Ukraine, Kiev
  • V. N. Barvinchenko Institute of Surface Chemistry named by A.A. Chuyko of the National Academy of Sciences of Ukraine, Kiev
  • N. P. Galagan Institute of Surface Chemistry named by A.A. Chuyko of the National Academy of Sciences of Ukraine, Kiev
  • I. V. Siora Institute of Surface Chemistry named by A.A. Chuyko of the National Academy of Sciences of Ukraine, Kiev
  • V. V. Turov Institute of Surface Chemistry named by A.A. Chuyko of the National Academy of Sciences of Ukraine, Kiev

Keywords:

cryopreservation, cryoprotectants, NMR-spectroscopy, hydration ability, bound water, interfacial energy

Abstract

Protein molecules’ hydration in the presence of sugars: glucose, fructose and sucrose were studied by NMR-spectroscopy. The parameters of hydration, concentration of bound water and value of interfacial energy were determined over a wide range of component concentrations. It was shown with exemplifying using the serum albumin, that low-molecular compounds (sugars) could replace an intraglobular water. The maximal effect of dehydration was observed for sucrose. In this case protein molecules change their property and capability to be adsorbed on a surface of fine-grained silica, which is reduced by 25% as a result of the forming complexes.

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Published

2007-12-15

How to Cite

Rugal, A. A., Barvinchenko, V. N., Galagan, N. P., Siora, I. V., & Turov, V. V. (2007). Effect of Sugars on Dehydration of Biopolymer Molecules During Cryopreservation. Problems of Cryobiology and Cryomedicine, 17(4), 374–384. Retrieved from https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/484

Issue

Vol. 17 No. 4 (2007): Problems of Cryobiology

Section

Theoretical and Experimental Cryobiology

License

Copyright (c) 2020 A. A. Rugal, V. N. Barvinchenko, N. P. Galagan, I. V. Siora, V. V. Turov

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