Effect of Cryopreserved Placental Explants on Female Reproductive System Under Normal and Pathological Conditions (Experimental study)

Authors

  • Volodymyr Yu. Prokopyuk Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv
  • Olga V. Grischenko V.N. Karazin Kharkiv National University, Kharkiv
  • Oleksandra V. Prokopyuk Kharkiv Medical Academy of Postgraduate Education, Kharkiv
  • Nadiia O. Shevchenko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv
  • Oksana V. Falko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv
  • Anna V. Storchak V.N. Karazin Kharkiv National University, Kharkiv
  • Andrii O. Schedrov Kharkiv State Medical University, Kharkiv

DOI:

https://doi.org/10.15407/cryo27.03.250

Keywords:

placenta, cryopreservation, mice, rats, pregnancy, infertility, antiphospholipid syndrome, polycystic ovary syndrome, infection, ovarian torsion

Abstract

One of the most important tasks in current medicine is the preservation and restoration of fertility. The application of human placental derivatives (mesenchymal stromal cells, fetal membranes, extracts and explants), having a high proliferative potential and the affinity to female reproductive system, is a promising direction for treating female reproductive pathology. Here we determined the effect of cryopreserved placental explants on female reproductive system under normal condi-tions (fertility and estral cycle) and in pathology (infectious process, endocrinopathy, autoimmune pathology, trauma/ischemia). The cryopreserved placental explants were revealed to have a trophic effect on uterus and ovaries, they temporarily delayed ovulation and positively affected autoimmune, endocrine pathology and ischemia course. In addition, placental explants aggravated the infection course and increased the peritoneal adhesion formation.

Probl Cryobiol Cryomed 2017; 27(3): 250–265

Author Biographies

Volodymyr Yu. Prokopyuk, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cryobiology of Reproductive System

Nadiia O. Shevchenko, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cryobiology of Reproductive System

Oksana V. Falko, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Low Temperature Bank of Biological Objects

References

Cole L.A. Biological functions of hCG and hCG-related molecules. Reprod Biol Endocrinol 2010; 8: 102. CrossRef PubMed

Guan-Yu X., I-Hsuan L., Chun-Chun C. et al. Amniotic fluid stem cells prevent follicle atresia and rescue fertility of mice with premature ovarian failure induced by chemotherapy. PLoS One 2014; 9(9): e106538. CrossRef PubMed

Han N.R., Park C.L., Kim N.R. et al. Protective effect of porcine placenta in a menopausal ovariectomized mouse. Reproduction 2015; 150(3): 173–181. CrossRef PubMed

Hanson B., Johnstone E., Dorais J. et al. Female infertility, infertilityassociated diagnoses, and comorbidities: a review. J Assist Reprod Genet 2016; 34(2): 167–177. CrossRef PubMed

Kulakov V.I., Adamyan L.V., Mynbayev O.A. Postoperative adhesions: etiology, pathogenesis, prevention. Moscow: Meditsina; 1998.

Lee J.M., Jung J., Lee H.J. et al. Comparison of immunomodulatory effects of placenta mesenchymal stem cells with bone marrow and adipose mesenchymal stem cells. Int Immunopharmacol 2012; 13(2): 219–224. CrossRef PubMed

Lee Y.K., Chung H.H., Kang S.B. et al. Efficacy and safety of human placenta extract in alleviating climacteric symptoms: prospective, randomized, double-blind, placebo-controlled trial. J Obstet Gynaecol Res 2009; 35(6): 1096–1101. CrossRef PubMed

Nasadyuk K.M. Cell technologies in reproductology, obstetrics and gynecology. Cell and Organ Transplantology 2013; 1(1): 61–65. CrossRef

Pandey V., Singh A., Singh A. et al. Role of oxidative stress and low-grade inflammation in letrozole-induced polycystic ovary syndrome in the rat. Reprod Biol 2016; 16(1): 70–77. CrossRef PubMed

Pipino C., Shangaris P., Resca E. et al. Placenta as a reservoir of stem cells: an underutilized resource? Br Med Bull 2013; 105: 43–68. CrossRef PubMed

Prokopyuk O.S., Prokopyuk V.Yu., Pasieshvili N.M. et al. Implantation of cryopreserved human placental fragments restores prooxidant-antioxidant balance in experimental animals of late ontogeny. Probl Cryobiol Cryomed 2017; 27(1): 61–70. CrossRef

Prokopyuk V.Yu., Prokopyuk O.S., Musatova I.B. et al. Safety of placental, umbilical cord and fetal membrane explants after cryopreservation. Cell and Organ Transplantology 2015; 3(1): 34–38. CrossRef

Rittirsch D., Huber-Lang M.S., Flierl M.A., Ward P.A. Immunodesign of experimental sepsis by cecal ligation and puncture. Nat Protoc 2009; 4(1): 31–36. CrossRef PubMed

Ruiz A., Aguilar R., Tebar M. et al. RU486-treated rats show endocrine and morphological responses to therapies analogous to responses of women with polycystic ovary syndrome treated with similar therapies. Biol Reprod 1996; 55(6): 1284–1291. CrossRef PubMed

Schevchenko N.O., Somova K.V., Prokopyuk V.Yu. et al. Dynamics of activity and duration of functioning of cryopreserved cryoextract, placental cells and fragments in the organism of experimental animals. Morphologia 2016; 10(2): 93–98.

Silini A.R., Cargnoni A., Magatti M. et al. The long path of human placenta, and its derivatives, in regenerative medicine. Front Bioeng Biotechnol 2015; 3: 162. CrossRef PubMed

Stefanov O.V., editor. Preclinical trials of medicines [guidelines]. Kyiv: Avitsena; 2001.

Taskin O, Birincioglu M, Aydin A. et al. The effects of twisted ischaemic adnexa managed by detorsion on ovarian viability and histology: an ischaemia-reperfusion rodent model. Hum Reprod 1998; 13(10): 2823–2827. CrossRef PubMed

Trifonov V.Y., Prokopyuk V.Y., Zaychenko A.V. Cryopreserved cord blood serum for reproductive function restoration during antiphospholipid syndrome. Probl Cryobiol 2011: 21(1), 75–84.

Veenstra van Nieuwenhoven A.L., Heineman M.J., Faas M.M. The immunology of successful pregnancy. Hum Reprod 2003; 9(4): 347–57. CrossRef

Velayuthaprabhu S., Archunan G., Balakrishnan K. Placental thrombosis in experimental anticardiolipin antibodies-mediated intrauterine fetal death. Am J Reprod Immunol 2007; 57(4): 270–276. CrossRef PubMed

Zarinara A., Zeraati H., Kamali K. et al. Models predicting success of infertility treatment: a systematic review. J Reprod Infertil 2016; 17(2): 68–81. PubMed

Downloads

Published

2017-09-25

How to Cite

Prokopyuk, V. Y., Grischenko, O. V., Prokopyuk, O. V., Shevchenko, N. O., Falko, O. V., Storchak, A. V., & Schedrov, A. O. (2017). Effect of Cryopreserved Placental Explants on Female Reproductive System Under Normal and Pathological Conditions (Experimental study). Problems of Cryobiology and Cryomedicine, 27(3), 250–265. https://doi.org/10.15407/cryo27.03.250

Issue

Vol. 27 No. 3 (2017): Probl Cryobiol Cryomed

Section

Cryomedicine, Clinical and Experimental Transplantology

License

Authors who publish with this journal agree to the following terms:

  • Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
  • Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
  • Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).