Low-Temperature Storage of Placenta Affects Anti-Inflammatory and Wound Healing Properties of its Extracts
DOI:
https://doi.org/10.15407/cryo32.02.144Keywords:
low-temperature preservation, human placenta, aqueous-saline extracts, erythrocyte thermohemolysis, platelet aggregation, burns, wound healing effectAbstract
Here we have studied the effect of low-temperature storage of the placenta at different temperatures on anti-inflammatory activity of its extracts in cell models of erythrocyte thermohemolysis and adenosine-5’-diphosphate-induced platelet aggregation. The wound-healing effect of cryopreserved placental extracts was also evaluated in rat thermal burn model. When preserving placenta for up to a month, the temperature of storage was shown to cause no significant impact on the percentage of inhibition of erythrocyte thermohemolysis and platelet aggregation by the extracts. Placental storage for three months at –20°C decreased anti-inflammatory activity of its extracts, and led to its complete loss during 6 months. Placental cryopreservation at –196°C enabled to preserve the anti-inflammatory and wound-healing activity. The cryopreserved placenta-derived extracts showed a pronounced positive effect on reparative process at third-degree (B) skin burn injury in rats.
Probl Cryobiol Cryomed 2022; 32(2):144–157
References
Alkadi KAA, Adam A, Muhammadtaha MH, et al. Antiplatelet aggregation activity of 5-hydroxyfl avone, 2'- hydroxyfl avanone, paeonol and bergenin isolated from stem bark of Garcinia malaccensis in human whole blood. Orient J Chem. 2013; 29(3): 871-5. CrossRef
Anosike CA, Obidoa O, Ezeanyika LU. Membrane stabilization as a mechanism of the anti-infl ammatory activity of methanol extract of garden egg (Solanum aethiopicum). DARU J Pharm Sci. 2012 Nov 14 [cited June 15 2021]; 20(1): 76. Available from: https://www.ncbi.nlm.nih.gov/pmc/articles/ PMC3556049/ CrossRef
Banerjee KK, Bishayee A, Chatterjee M. Anti-inflammatory effect of human placental extract: a biochemical mechanistic approach. Riv Eur Sci Med Farmacol. 1992; 14(6): 361- 6. PubMed
Banerjee KK, Bishayee A, Chatterjee M. Role of human placental extract on succinic dehydrogenase activity in carrageenin-induced edema in rats in vivo and its effect on erythrocyte lysis, platelet aggregation and trypsin activity in vitro. Indian J Physiol Pharmacol. 1994; 38(2): 121-4. PubMed
Bezhin AI, Pankrusheva TA, Grigoryan AY, et al. Miramistinum and metronidazole in the local treatment of the experimental purulent wounds. RJPBCS. 2014; 5(4): 1677- 85.
Bhadoriya SS, Mishra V, Raut S, et al. Anti-inflammatory and antinociceptive activities of a hydroethanolic extract of Tamarindus indica leaves. Sci Pharm. 2012; 80(3): 685-700. CrossRef
Biswas TK, Auddy B, Bhattacharya NP, et al. Wound healing activity of human placental extract in rats. Acta Pharmacologica Sinica. 2001; 22(12): 1113- 6. PubMed
Bougandoura A, D'Abrosca B, Ameddah S, et al. Chemical constituents and in vitro anti-inflammatory activity of Cistanche violacea Desf. (Orobanchaceae) extract. Fitoterapia. 2016; 109(2): 248-53. CrossRef
Bynum JA, Taylor AS, Peltier GC, et al. Evaluation of adenosine, lidocaine, and magnesium for enhancement of platelet function during storage. J Trauma Acute Care Surg. 2017; 83(1): S9-S15. CrossRef
Chakraborty PD, De D, Bandyopadhyay S, Bhattacharyya D. Human aqueous placental extract as a wound healer. J Wound Care. 2009; 18(11): 462-7. CrossRef
Chang AC, Dearman B, Greenwood JE. A comparison of wound area measurement techniques: visitrak versus photography. Eplasty. [Internet]. 2011 Apr 18 [cited June 15 2021]; 11: e18. Available from:
https://www.hmpgloballearningnetwork.com/site/eplasty/comparison-wound-area-measurement-techniques-visitrak-versus-photography PubMed
Choi JS, Kim JD, Yoon HS, Cho YW. Full-thickness skin wound healing using human placenta-derived extracellular matrix containing bioactive. Molecules Tissue Eng. Part A. 2013; 19(3-4): 329-39. CrossRef
Ding F, Deng H, Du Y, et al. Emerging chitin and chitosan nanofibrous materials for biomedical applications. Nanoscale. 2014; 6: 9477-93. CrossRef
Gilpin DA. Calculation of a new Meeh constant and experimental determination of burn size. Burns. 1996; 22(8): 607- 11. CrossRef
Hong JW, Lee WJ, Hahn SB, et al. The effect of human placenta extract in a wound healing model. Ann Plast Surg. 2010; 65(1): 96-100. CrossRef
Jazayeri MH, Вarzaman K, Nedaeinia R, et al. Human placental extract attenuates neurological symptoms in the experimental autoimmune encephalomyelitis model of multiple sclerosis - a putative approach in MS disease? Auto Immun Highlights. [Internet]. 2020 Oct 4 [cited June 15 2021]; 11(1): 14. Available from: https://autoimmunhighlights.biomedcentral.com/articles/10.1186/s13317-020-00137-x CrossRef
Jimi S, Kimura M, Francesco FD, et al. Acceleration mechanisms of skin wound healing by autologous micrograft in mice. Int J Mol Sci. [Internet]. 2017 Aug 2 [cited June 10 2021]; 18(8): 1675. Available from: https://www.mdpi.com/1422-0067/18/8/1675/htm CrossRef
Joshi MG, Kshersagar J, Desai SR, Sharma S. Antiviral properties of placental growth factors: A novel therapeutic approach for COVID-19 treatment. Placenta. 2020; 99: 117-30. CrossRef
Katkurwar A, Chaudhari D, Mahale S, et al. Human placental extract a miracle that heals the wound faster. J Oral Res Rev. 2021; 13(1): 1-5. CrossRef
Kottke-Marchant K, Corcoran G. The laboratory diagnosis of platelet disorders: an algorithmic approach. Arch Pathol Lab Med. 2002. 126: 133-46. CrossRef
Kurahashi T, Fujii J. Roles of antioxidative enzymes in wound healing. J Dev Biol. 2015; 3: 57-70. CrossRef
Mao Y, Singh-Varma A, Hoffman T, et al. The effect of cryopreserved human placental tissues on biofilm formation of wound-associated pathogen. J Funct Biomater. [Internet]. 2018 Jan 8 [cited June 14 2021]; 9(1): 3. Available from: https://www.mdpi.com/2079-4983/9/1/3/htm CrossRef
Meijer K, Vonk RJ, Priebe MG, Roelofsen H. Cell-based screening assay for anti-inflammatory activity of bioactive compounds. Food Chem. 2015: 166: 158-64. CrossRef
Mukherjee C, Saleem S, Das S, et al. Human placental laminin: Role in neuronal differentiation, cell adhesion and proliferation. J Biosci. [Internet]. 2020 July 2 [cited June 14 2021]; 45: 93. Available from: https://www.ias.ac.in/article/fulltext/jbsc/045/0093 CrossRef
Nagae M, Nagata M, Teramoto M, et al. Effect of porcine placenta extract supplement on skin condition in healthy adult women: a randomized, double-blind placebocontrolled study. Nutrients. [Internet]. 2020 June 4 [cited June 10 2021]; 12(6): 1671. Available from: https://www.mdpi.com/2072-6643/12/6/1671/htm CrossRef
Nakamura H, Yokoyama Y, Motoyoshi S, et al. Effect of a nonsteroidal anti-inflammatory agent, tolmetin sodium on exudative inflammation in experimental animals. Nippon Yakurigaku Zasshi. 1979; 75(5): 447-58. CrossRef
Nardid OA, Repina SV, Rozanova ED, et al. Properties of aqueous-saline human placental extracts and their fractions after storage of placenta at various subzero temperatures. J Exp Integr Med. 2015; 5(4): 172- 7. CrossRef
Narozhnyi SV, Rozanova KD, Bobrova OM, Nardid OA. Antioxidant and antiradical effects of extracts derived from cryopreserved human placenta. Probl Cryobiol Cryomed. 2018; 28(4): 323-32. CrossRef
Nath S, Bhattacharyya D. Cell adhesion by aqueous extract of human placenta used as wound healer. Indian J Exp Biol. 2007; 45(8): 732-8. PubMed
Okoli CO, Akah PA, Onuoha NJ, et al. Acanthus montanus: an experimental evaluation of the antimicrobial, anti-inflammatory and immunological properties of a traditional remedy for furuncles. BMC Complement Altern Med. [Internet]. 2008 June 6 [cited June 11 2021]; 8: 27. Available from: https://bmccomplementmedtherapies.biomedcentral.com/articles/10.1186/1472-6882-8-27 CrossRef
Palanker ND, Lee CT, Weltman RL, et al. Antimicrobial efficacy assessment of human derived composite amnion-chorion membrane. Sci Rep. [Internet]. 2019 Oct 30 [cited June 8 2021]; 9: 15600. Available from: https://www.nature.com/articles/s41598-019-52150-4 CrossRef
Pan SY, Chan MKS, Wong MBF, et al. Placental therapy: an insight to their biological and therapeutic properties. J Med Therap. 2017; 1(3): 1-6. CrossRef
Park H-J, Shim HS, Lee S, et al. Anti-stress effects of human placenta extract: possible involvement of the oxidative stress system in rats. BMC Complement Altern Med. [Internet]. 2018 May 08 [cited June 7 2021]; 18: 149. Available from: https://bmccomplementmedtherapies.biomedcentral.com/articles/10.1186/s12906-018-2193-x CrossRef
Pogozhykh DN, Rozanova ED, Nardid OA. Change of properties of human placenta aqueous-saline extracts during low temperature storage. Problems of Cryobiology. 2008; 18(1): 22-6. Full Text
Rozanova SL, Naumenko YeI, Nardid EO. Influence of low temperature storage and ultrasonic treatment of placenta on its extracts antioxidant properties. Probl Cryobiol Cryomed. 2015; 25(3): 255-66. CrossRef
Rozanova SL, Rozanova ED, Nardid OA, Karpenko VG. Antioxidant activity of placenta extracts after low temperature and hypothermic storage. Problems of Cryobiology. 2011; 21(3): 291-300. Full Text
Sur TK, Biswas TK, Ali L, Mukherjee B. Anti-inflammatory and anti-platelet aggregation activity of human placental extract. Acta Pharmacol Sin. 2003; 24(2): 187- 92. PubMed
Yesmin S, Paul A, Naz T, et al. Membrane stabilization as a mechanism of the anti-inflammatory activity of ethanolic root extract of Choi (Piper chaba). Clin Phytosci. 2020 Aug 27 2020 [cited June 7 2021]; 6: 59. Available from: https://clinphytoscience.springeropen.com/articles/10.1186/s40816-020-00207-7 CrossRef
Zhang D, Lijuan G, Jingjie L, et al. Cow placenta extract promotes murine hair growth through enhancing the insulin-like growth factor-1. Indian J Dermatol. 2011; 56(1): 14-8. CrossRef
Downloads
Published
How to Cite
Issue
Section
License
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
