Low-Temperature Storage of Placenta Affects Anti-Inflammatory and Wound Healing Properties of its Extracts

Authors

  • Olena M. Bobrova Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv https://orcid.org/0000-0001-9100-1823
  • Yevheniia Yo. Naumenko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv https://orcid.org/0000-0002-9727-3165
  • Myroslav I. Shchetinskyi Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv https://orcid.org/0000-0002-9279-2695
  • Stanislav V. Narozhnyi Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv https://orcid.org/0000-0001-9690-0203
  • Оleg А. Nardid 1 Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv; 2 V. N. Karazin Kharkiv National University, Kharkiv https://orcid.org/0000-0002-9439-0606
  • Мarina М. Kalashnykova Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv https://orcid.org/0000-0001-7813-7208
  • Irina I. Shchetinska National Technical University ‘Kharkiv Polytechnic Institute’, Kharkiv

DOI:

https://doi.org/10.15407/cryo32.02.144

Keywords:

low-temperature preservation, human placenta, aqueous-saline extracts, erythrocyte thermohemolysis, platelet aggregation, burns, wound healing effect

Abstract

Here we have studied the effect of low-temperature storage of the placenta at different temperatures on anti-inflammatory activity of its extracts in cell models of erythrocyte thermohemolysis and adenosine-5’-diphosphate-induced platelet aggregation. The wound-healing effect of cryopreserved placental extracts was also evaluated in rat thermal burn model. When preserving placenta for up to a month, the temperature of storage was shown to cause no significant impact on the percentage of inhibition of erythrocyte thermohemolysis and platelet aggregation by the extracts. Placental storage for three months at –20°C decreased anti-inflammatory activity of its extracts, and led to its complete loss during 6 months. Placental cryopreservation at –196°C enabled to preserve the anti-inflammatory and wound-healing activity. The cryopreserved placenta-derived extracts showed a pronounced positive effect on reparative process at third-degree (B) skin burn injury in rats.

Probl Cryobiol Cryomed 2022; 32(2):144157

Author Biographies

Olena M. Bobrova, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cryobiophysics

Yevheniia Yo. Naumenko , Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cryobiophysics

Myroslav I. Shchetinskyi , Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cryobiophysics

Stanislav V. Narozhnyi , Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cryobiophysics

Оleg А. Nardid , 1 Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv; 2 V. N. Karazin Kharkiv National University, Kharkiv

Department of Cryobiophysics

Мarina М. Kalashnykova , Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cryobiophysics

References

Alkadi KAA, Adam A, Muhammadtaha MH, et al. Antiplatelet aggregation activity of 5-hydroxyfl avone, 2'- hydroxyfl avanone, paeonol and bergenin isolated from stem bark of Garcinia malaccensis in human whole blood. Orient J Chem. 2013; 29(3): 871-5. CrossRef

Anosike CA, Obidoa O, Ezeanyika LU. Membrane stabilization as a mechanism of the anti-infl ammatory activity of methanol extract of garden egg (Solanum aethiopicum). DARU J Pharm Sci. 2012 Nov 14 [cited June 15 2021]; 20(1): 76. Available from: https://www.ncbi.nlm.nih.gov/pmc/articles/ PMC3556049/ CrossRef

Banerjee KK, Bishayee A, Chatterjee M. Anti-inflammatory effect of human placental extract: a biochemical mechanistic approach. Riv Eur Sci Med Farmacol. 1992; 14(6): 361- 6. PubMed

Banerjee KK, Bishayee A, Chatterjee M. Role of human placental extract on succinic dehydrogenase activity in carrageenin-induced edema in rats in vivo and its effect on erythrocyte lysis, platelet aggregation and trypsin activity in vitro. Indian J Physiol Pharmacol. 1994; 38(2): 121-4. PubMed

Bezhin AI, Pankrusheva TA, Grigoryan AY, et al. Miramistinum and metronidazole in the local treatment of the experimental purulent wounds. RJPBCS. 2014; 5(4): 1677- 85.

Bhadoriya SS, Mishra V, Raut S, et al. Anti-inflammatory and antinociceptive activities of a hydroethanolic extract of Tamarindus indica leaves. Sci Pharm. 2012; 80(3): 685-700. CrossRef

Biswas TK, Auddy B, Bhattacharya NP, et al. Wound healing activity of human placental extract in rats. Acta Pharmacologica Sinica. 2001; 22(12): 1113- 6. PubMed

Bougandoura A, D'Abrosca B, Ameddah S, et al. Chemical constituents and in vitro anti-inflammatory activity of Cistanche violacea Desf. (Orobanchaceae) extract. Fitoterapia. 2016; 109(2): 248-53. CrossRef

Bynum JA, Taylor AS, Peltier GC, et al. Evaluation of adenosine, lidocaine, and magnesium for enhancement of platelet function during storage. J Trauma Acute Care Surg. 2017; 83(1): S9-S15. CrossRef

Chakraborty PD, De D, Bandyopadhyay S, Bhattacharyya D. Human aqueous placental extract as a wound healer. J Wound Care. 2009; 18(11): 462-7. CrossRef

Chang AC, Dearman B, Greenwood JE. A comparison of wound area measurement techniques: visitrak versus photography. Eplasty. [Internet]. 2011 Apr 18 [cited June 15 2021]; 11: e18. Available from:

https://www.hmpgloballearningnetwork.com/site/eplasty/comparison-wound-area-measurement-techniques-visitrak-versus-photography PubMed

Choi JS, Kim JD, Yoon HS, Cho YW. Full-thickness skin wound healing using human placenta-derived extracellular matrix containing bioactive. Molecules Tissue Eng. Part A. 2013; 19(3-4): 329-39. CrossRef

Ding F, Deng H, Du Y, et al. Emerging chitin and chitosan nanofibrous materials for biomedical applications. Nanoscale. 2014; 6: 9477-93. CrossRef

Gilpin DA. Calculation of a new Meeh constant and experimental determination of burn size. Burns. 1996; 22(8): 607- 11. CrossRef

Hong JW, Lee WJ, Hahn SB, et al. The effect of human placenta extract in a wound healing model. Ann Plast Surg. 2010; 65(1): 96-100. CrossRef

Jazayeri MH, Вarzaman K, Nedaeinia R, et al. Human placental extract attenuates neurological symptoms in the experimental autoimmune encephalomyelitis model of multiple sclerosis - a putative approach in MS disease? Auto Immun Highlights. [Internet]. 2020 Oct 4 [cited June 15 2021]; 11(1): 14. Available from: https://autoimmunhighlights.biomedcentral.com/articles/10.1186/s13317-020-00137-x CrossRef

Jimi S, Kimura M, Francesco FD, et al. Acceleration mechanisms of skin wound healing by autologous micrograft in mice. Int J Mol Sci. [Internet]. 2017 Aug 2 [cited June 10 2021]; 18(8): 1675. Available from: https://www.mdpi.com/1422-0067/18/8/1675/htm CrossRef

Joshi MG, Kshersagar J, Desai SR, Sharma S. Antiviral properties of placental growth factors: A novel therapeutic approach for COVID-19 treatment. Placenta. 2020; 99: 117-30. CrossRef

Katkurwar A, Chaudhari D, Mahale S, et al. Human placental extract a miracle that heals the wound faster. J Oral Res Rev. 2021; 13(1): 1-5. CrossRef

Kottke-Marchant K, Corcoran G. The laboratory diagnosis of platelet disorders: an algorithmic approach. Arch Pathol Lab Med. 2002. 126: 133-46. CrossRef

Kurahashi T, Fujii J. Roles of antioxidative enzymes in wound healing. J Dev Biol. 2015; 3: 57-70. CrossRef

Mao Y, Singh-Varma A, Hoffman T, et al. The effect of cryopreserved human placental tissues on biofilm formation of wound-associated pathogen. J Funct Biomater. [Internet]. 2018 Jan 8 [cited June 14 2021]; 9(1): 3. Available from: https://www.mdpi.com/2079-4983/9/1/3/htm CrossRef

Meijer K, Vonk RJ, Priebe MG, Roelofsen H. Cell-based screening assay for anti-inflammatory activity of bioactive compounds. Food Chem. 2015: 166: 158-64. CrossRef

Mukherjee C, Saleem S, Das S, et al. Human placental laminin: Role in neuronal differentiation, cell adhesion and proliferation. J Biosci. [Internet]. 2020 July 2 [cited June 14 2021]; 45: 93. Available from: https://www.ias.ac.in/article/fulltext/jbsc/045/0093 CrossRef

Nagae M, Nagata M, Teramoto M, et al. Effect of porcine placenta extract supplement on skin condition in healthy adult women: a randomized, double-blind placebocontrolled study. Nutrients. [Internet]. 2020 June 4 [cited June 10 2021]; 12(6): 1671. Available from: https://www.mdpi.com/2072-6643/12/6/1671/htm CrossRef

Nakamura H, Yokoyama Y, Motoyoshi S, et al. Effect of a nonsteroidal anti-inflammatory agent, tolmetin sodium on exudative inflammation in experimental animals. Nippon Yakurigaku Zasshi. 1979; 75(5): 447-58. CrossRef

Nardid OA, Repina SV, Rozanova ED, et al. Properties of aqueous-saline human placental extracts and their fractions after storage of placenta at various subzero temperatures. J Exp Integr Med. 2015; 5(4): 172- 7. CrossRef

Narozhnyi SV, Rozanova KD, Bobrova OM, Nardid OA. Antioxidant and antiradical effects of extracts derived from cryopreserved human placenta. Probl Cryobiol Cryomed. 2018; 28(4): 323-32. CrossRef

Nath S, Bhattacharyya D. Cell adhesion by aqueous extract of human placenta used as wound healer. Indian J Exp Biol. 2007; 45(8): 732-8. PubMed

Okoli CO, Akah PA, Onuoha NJ, et al. Acanthus montanus: an experimental evaluation of the antimicrobial, anti-inflammatory and immunological properties of a traditional remedy for furuncles. BMC Complement Altern Med. [Internet]. 2008 June 6 [cited June 11 2021]; 8: 27. Available from: https://bmccomplementmedtherapies.biomedcentral.com/articles/10.1186/1472-6882-8-27 CrossRef

Palanker ND, Lee CT, Weltman RL, et al. Antimicrobial efficacy assessment of human derived composite amnion-chorion membrane. Sci Rep. [Internet]. 2019 Oct 30 [cited June 8 2021]; 9: 15600. Available from: https://www.nature.com/articles/s41598-019-52150-4 CrossRef

Pan SY, Chan MKS, Wong MBF, et al. Placental therapy: an insight to their biological and therapeutic properties. J Med Therap. 2017; 1(3): 1-6. CrossRef

Park H-J, Shim HS, Lee S, et al. Anti-stress effects of human placenta extract: possible involvement of the oxidative stress system in rats. BMC Complement Altern Med. [Internet]. 2018 May 08 [cited June 7 2021]; 18: 149. Available from: https://bmccomplementmedtherapies.biomedcentral.com/articles/10.1186/s12906-018-2193-x CrossRef

Pogozhykh DN, Rozanova ED, Nardid OA. Change of properties of human placenta aqueous-saline extracts during low temperature storage. Problems of Cryobiology. 2008; 18(1): 22-6. Full Text

Rozanova SL, Naumenko YeI, Nardid EO. Influence of low temperature storage and ultrasonic treatment of placenta on its extracts antioxidant properties. Probl Cryobiol Cryomed. 2015; 25(3): 255-66. CrossRef

Rozanova SL, Rozanova ED, Nardid OA, Karpenko VG. Antioxidant activity of placenta extracts after low temperature and hypothermic storage. Problems of Cryobiology. 2011; 21(3): 291-300. Full Text

Sur TK, Biswas TK, Ali L, Mukherjee B. Anti-inflammatory and anti-platelet aggregation activity of human placental extract. Acta Pharmacol Sin. 2003; 24(2): 187- 92. PubMed

Yesmin S, Paul A, Naz T, et al. Membrane stabilization as a mechanism of the anti-inflammatory activity of ethanolic root extract of Choi (Piper chaba). Clin Phytosci. 2020 Aug 27 2020 [cited June 7 2021]; 6: 59. Available from: https://clinphytoscience.springeropen.com/articles/10.1186/s40816-020-00207-7 CrossRef

Zhang D, Lijuan G, Jingjie L, et al. Cow placenta extract promotes murine hair growth through enhancing the insulin-like growth factor-1. Indian J Dermatol. 2011; 56(1): 14-8. CrossRef

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Published

2022-10-30

How to Cite

Bobrova, O., Naumenko , Y., Shchetinskyi , M., Narozhnyi , S., Nardid О. ., Kalashnykova М., & Shchetinska , I. . (2022). Low-Temperature Storage of Placenta Affects Anti-Inflammatory and Wound Healing Properties of its Extracts . Problems of Cryobiology and Cryomedicine, 32(2), 144–157. https://doi.org/10.15407/cryo32.02.144

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Section

Cryomedicine, Clinical and Experimental Transplantology