Influence of Freezing Regimens on Cord Blood Serum Dielectric Properties

Authors

  • O. A. Gorobchenko V.N. Karazin Kharkov National University, Kharkov
  • Yu. A. Moshko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • O. T. Nikolov V.N. Karazin Kharkov National University, Kharkov
  • O. A. Nardid Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • O. V. Lipina Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov
  • S. V. Gatash Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkov

Keywords:

cord blood, serum, cryopreservation, UHF-dielectrometry, dielectric permittivity

Abstract

Using UHF dielectrometry method the temperature dependencies of the complex dielectric permittivity for water molecules of native and frozen samples of cord blood serum (CBS) were investigated within the interval 4-40°C. For all the investigated samples non-monotonous changes in ε′ at 7-9, 16-20, 26-28 and 36-38°C were found. It is established that under slow freezing down to -20°C the serum ε′ value is lower than in both native samples and those subjected to rapid freezing, this may be the cause of increase in the amount of bound water in the system due to the growth of serum molecules hydration. Rapid freezing results in the ε′ increase in CBS, caused by cryoaggregation processes and dehydration of macromolecules in it.

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Published

2004-06-15

How to Cite

Gorobchenko, O. A., Moshko, Y. A., Nikolov, O. T., Nardid, O. A., Lipina, O. V., & Gatash, S. V. (2004). Influence of Freezing Regimens on Cord Blood Serum Dielectric Properties. Problems of Cryobiology and Cryomedicine, (2), 4–10. Retrieved from https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/682

Issue

No. 2 (2004): Problems of Cryobiology

Section

Theoretical and Experimental Cryobiology

License

Copyright (c) 2020 O. A. Gorobchenko, Yu. A. Moshko, O. T. Nikolov, O. A. Nardid, O. V. Lipina, S. V. Gatash

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