Structural and Functional Changes in Heart Tissues of Hetero- and Homeothermic Animals Under Artificial and Natural Hypometabolism

Authors

  • Viktoria V. Lomako Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv
  • Alexsandr V. Shilo Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv
  • Igor F. Kovalenko Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

DOI:

https://doi.org/10.15407/cryo26.04.308

Keywords:

hypometabolism, hibernation, homeothermy, heterothermy, myocardial histology, rats hamsters

Abstract

During artificial hypometabolism (combined effect of hypoxia, hypercapnia and hypothermia) there was a decrease in temperature of body (TB) down to (17 ± 1) and (16 ± 1)°C and heart rate down to (99 ± 20) and (66 ± 16) beats/min in rats and hamsters, respectively, and under hibernation it reduced down to (8 ± 1)°C and 15–13 beats/min. The hyperemia of veins, arteries and capillaries; increased perivascular and interstitial spaces; signs of grained, hydropic and hyaline-drop dystrophies (focally),as well as numerous nuclei at various necrobiosis stages (karyopyknosis, karyorhexis and karyolysis) were herewith observed in myocardial tissue. In addition, 2 hrs later artificial hypometabolism there were revealed the blood thrombi in vessels, nuclear-free zones of necrosis, and in rats even large nuclei in some cardiomyocytes. The changes persisted even 24 hrs later, but the most of those were reversible after TB and blood circulation recovery and had an adaptive nature, but the activation of necrobiotic processes was known to be promoted the acceleration of physiological regeneration.

Probl Cryobiol Cryomed 2016; 26(4): 308–321

Author Biographies

Viktoria V. Lomako, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cryophysiology

Alexsandr V. Shilo, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cryophysiology

Igor F. Kovalenko, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

Department of Cryophysiology

References

Bayevsky R.M., Ivanov G.G. Heart rate variability: theoretical aspects and clinical application. Moscow; 2000.

Barros R.C. H., Abe A.S., Carnio E.C., Branco L.G.S. Regulation of breathing and body temperature of a burrowing rodent during hypoxic-hypercapnia. Comp Biochem Physiol A Mol Integr Physiol. 2004; 138(1): 97–104. CrossRef PubMed

Blackstone E., Morrison M., Roth M.B. H2S induces a suspended animation-like state in mice. Science 2005: 308(5721): 518. CrossRef PubMed

Bouna H.R., Verhaag E.M., Otis J.P. et al. Induction of torpor: mimicking natural metabolic suppression for biomedical applications. J Cell Physiol 2012; 227(4): 1285–1290. CrossRef PubMed

Bradford M.M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of proteindye binding. Anal Biochem 1976; 72(7): 248–254. CrossRef

Carey H.V., Andrews M.T., Martin S.L. Mammalian hibernation: cellular and molecular responses to depressed metabolism and low temperature. Physiol Rev 2003; 83(4): 1153–1181. CrossRef PubMed

Chatfield P.O., Lyman C.P. Circulatory changes during process of arousal in the hibernating hamsters. Am J Physiol 1950; 163(3): 566–574. PubMed

de Vrij E.L., Vogelaar P.C., Goris M. et al. Platelet dynamics during natural and pharmacologically induced torpor and forced hypothermia. PLoS ONE 2014; 9(4): e93218. CrossRef PubMed

Deveci D., Egginton S. Differing mechanisms of cold-induced changes in capillary supply in m. tibialis anterior of rats and hamsters. J Experim Biology 2002; 205(6): 829–840.

Dickson B.A. Venous thrombosis: on the history of Virchow's triad. University of Toronto Medical Journal 2004; 81(3): 166–171.

Dikic D., Heldmaier G., Meyer C.W. Induced torpor in different strains of laboratory mice. In: Lovegrove B.G., McKechnie A.E., editors. Hypometabolism in animals: torpor, hibernation and cryobiology. Pietermaritzburg: University of KwaZulu-Natal; 2008. p. 223–230.

Drew K.L., Rice M.E., Kuhn T.B., Smith M.A. Neuroprotective adapatations in hibernation: therapeutic implications for ischemiareperfusion, traumatic brain injury and neurodegenerative diseases. Free Radic Biol Med 2001; 31(5): 563–573. CrossRef

Eagles D.A., Jacques L.B., Taboada J. et al. Cardiac arrhythmias during arousal from hibernation in three species of rodents. Am J Physiol 1988: 254(1): 102–108.

Field L. Modulation of the cardiomyocyte cell cycle in genetically altered animals. Ann N Y Acad Sci 2004; 1015: 160–170. CrossRef PubMed

Heldmaier G., Ortmann S., Elvert R. Natural hypometabolism during hibernation and daily torpor in mammals. Respir Physiol Neurobiol 2004; 141(3): 317–329. CrossRef PubMed

Horwitz B.A., Chau S.M., Hamilton J.S. et al. Temporal relationships of blood pressure, heart rate, baroreflex function, and body temperature change over a hibernation bout in Syrian hamsters. Am J Physiol Regul Integr Comp Physiol 2013; 305(7): R759–R768. CrossRef

Jarsky T.M., Stephenson R. Effects of hypoxia and hypercapnia on circadian rhythms in the golden hamster (Mesocricetus auratus). J Appl Physiol 2000; 89(6): 2130–2138.

Johansson B.W. The hibernator heart-nature's model of resis-tance to ventricular fibrillation. Cardiovasc Res 1996; 31: 826–832. CrossRef PubMed

Kuhnen G., Wloch B., Wunnenberg W. Effects of acute hypoxia and/or hypercapnia on body temperatures and cold induced thermogenesis in the golden hamster. J Therm Biol 1987; 12(2):103–107. CrossRef

Kоzlova V.F., Yurchenko Т.N. Structural aspects of adaptation in hibernators. Problems of Cryobiology 1996; (3): 44–51.

Lomako V.V., Samokhina L.M., Shylo O.V. Effect of natural and various artificial hypometabolism on activity of protease-protease inhibitor system in hamsters and rats. Problems of Cryobiology 2011; 21(3): 280–290.

Lomako V.V., Shylo A.V. Histological picture in neocortex and hypothalamus of homoio- and heterothermal animals under artificial and natural hipometabolism. Probl Cryobiol Cryomed 2015; (2): 93–103.

Lyman C.P., O'Brien R.C. Autonomic control of circulation during the hibernating cycle in ground squirrels. J Physiol (Lond) 1963; 168(3): 477–499. CrossRef

Melnichuk С.D., Меlnichuk D.О. Hypobiosis of animals (molecular mechanisms and practical implications for agriculture and medicine). Kyiv: Publishing Center NAU; 2007.

Mertens A., Stiedl O., Steinlechner S., Meyer M. Cardiac dynamics during daily torpor in the Djungarian hamster (Phodopus sungorus). Am J Physiol Regul Integr Comp Physiol 2008; 294 (2): R639–R650. CrossRef

Milsom W.K., Zimmer M.B., Harris M.B. Regulation of cardiac rhythm in hibernating mammals. Comp Biochem Physiol 1999; 124(4): 383–391. CrossRef

Nielsen K., Owman C. Difference in cardiac adrenergic innervation between hibernators and non-hibernating mammals. Acta Physiol Scand Suppl 1968; 316: 1–30. CrossRef

Phillips P.K., Heath J.E. Comparison of surface temperature in 13-lined ground squirrel (Spermophilus tridecimlineatus) and yellowbellied marmot (Marmota flaviventris) during arousal from hibernation. Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology 2004; 138(4): 451–457. CrossRef PubMed

Physiology of circulation. Physiology of blood. In a series: Physiology Guide. Leningrad: Nauka; 1980.

Polderman K.H., Herold I. Therapeutic hypothermia and controlled normothermia in the intensive care unit: practical considerations, side effects, and cooling methods. Crit Care Med 2009; 37(3):R1101–R1120. CrossRef

Reil J.-C., Custodis F., Swedberg K. et al. Heart rate reduction in cardiovascular disease and therapy. Clin Res Cardiol 2011; 100(1): 11–19. CrossRef PubMed

Shlyakhto E.V., Bokeria L.A., Rybakova M.G. et al. Cellular aspects of pathogenesis of hypertrophic cardiomyopathy: the role of cardiomyocyte polyploidy and activation of proliferating cell nuclear antigen in the myocardium. Tsitologiya 2007; 49(10):817–823. CrossRef

Shylo A.V., Lomako V.V., Samokhina L.M., Babijchuk G.A. Proteinases and its inhibitors activity at artificial hypometabolic state in rats. Problems of Cryobiology 2004; (2): 17–27.

Shylo O.V. Change of heart activity at artificial hypometabolic state and in the course of arousal in hibernators and nonhibernators. Naukovyy Visnyk NAU 2008: (126): 81–87.

Shylo O.V., Lomako V.V., Babiychuk G.O. Artificial hibernationcaused cardiac arrhythmia in homoio- and heterothermal animals. Cardiology of Uzbekistan 2016: 1–2 (39–40): 271–272.

Swoap S.J., Gutilla M.J. Cardiovascular changes during daily torpor in the laboratory mouse. Am J Physiol Regul Integr Comp Physiol 2009; 297 (Issue 3): R769–R774.

Timofeyev N.N., Prokof'eva L.P. Neurochemistry of hypobiosis and limits of organism cryoresistance. Ðœoscow: Meditsina; 1997.

Volkova О.V., Eletskiy Yu.K. Fundamentals of histology and histological techniques. Moscow: Meditsina; 1982.

Zhegunov G.F. Adaptation peculiarities of hibernators' heart. Problems of Cryobiology 1993; (3): 21–33.

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Published

2016-12-23

How to Cite

Lomako, V. V., Shilo, A. V., & Kovalenko, I. F. (2016). Structural and Functional Changes in Heart Tissues of Hetero- and Homeothermic Animals Under Artificial and Natural Hypometabolism. Problems of Cryobiology and Cryomedicine, 26(4), 308–321. https://doi.org/10.15407/cryo26.04.308

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Section

Theoretical and Experimental Cryobiology