Cryopreservation of L929 cell culture in protective solutions containing hyaluronic acid

Авторы

Ключевые слова:

cryopreservation, cryoprotectants, L929 cell culture, dimethyl sulfoxide, hyaluronic acid, cell adhesion

Аннотация

Probl Cryobiol Cryomed. 2026; 36(1): 27—31

Биографии авторов

Anatolii Seliuta, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv, Ukraine

Department of Low Temperature Preservation

Yevgeniya Smolyaninova, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv, Ukraine

Department of Low Temperature Preservation

Svitlana Kovalenko, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv, Ukraine

Department of Low Temperature Preservation

Olena Tymofieieva, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv, Ukraine

Department of Low Temperature Preservation

Hanna Poliakova, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv, Ukraine

Department of Low Temperature Preservation

Tetiana Gurina, Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv, Ukraine

Department of Low Temperature Preservation

Библиографические ссылки

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Garantziotis S, Savani RC. Hyaluronan biology: A complex balancing act of structure, function, location and context. Matrix Biol. 2019; 78-79: 1-10. CrossRef

Gurina TM, Kovalov GO, Polyakova AL, et al. Assessment of hyaluronic acid and DMSO influence on low-temperature tissue damage course: an experimental model. Probl Cryobiol Criomed. 2025; 35(4): 208-18. CrossRef

Gurina TM, Nardid EO, Seliuta AA, et al. Sterilization and low temperature effects on regenerative potential of hyaluronic acid. Probl Cryobiol Criomed. 2025; 35(2): 68-75. CrossRef

Gurruchaga H, Saenz del Burgoa L, Orivea G, et.al. Low molecular-weight hyaluronan as a cryoprotectant for the storage of microencapsulated cells. Int J Pharm. 2018; 518: 206-16. CrossRef

Jiang B, Li W, Stewart S, Ou W, et al. Sand-mediated ice seeding enables serum-free low-cryoprotectant cryopreservation of human induced pluripotent stem cells. Bioact Mater. 2021; 6(12): 4377-88. CrossRef

Liu M, Chen C, Yu J, et al. The gelatin-based liquid marbles for cell cryopreservation. Mater Today Bio [Internet]. 2022 Oct 31 [cited 2025 Nov 24]; 17: 100477. Available from: https://www.sciencedirect.com/science/article/pii/S2590006422002757 CrossRef

Matsumura K, Hayashi F, Nagashima T, et al. Molecular mechanisms of cell cryopreservation with polyampholytes studied by solid-state NMR. Commun Mater [Internet]. 2021 Feb 09 [cited 2025 Nov 24]; 2: 15. Available from: https://www.nature.com/articles/s43246-021-00118-1 CrossRef

Matsumura K, Bae JY, Hyon SH. Polyampholytes as cryoprotective agents for mammalian cell cryopreservation. Cell Transplant. 2010; 19: 691-9. CrossRef

Pakhomov O, Shevchenko N, Chernobai N, et al. Open-source hardware- and software-based cryomicroscopy system for investigation of phase transitions in cryobiological research. J. Microsc. 2024; 293(2): 71-85. CrossRef

Palasz A, Alkemade S, Mapletoft RJ. Use of sodium hyaluronate in freezing media for ovine and murine embryos. Cryobiology.1993; 30: 172-8. CrossRef

Pilbauerova N, Schmidt J, Soukup T, et al. Innovative approach in the cryogenic freezing medium for mesenchymal stem cells. Biomolecules [Internet]. 2022 Apr 20 [cited 2025 Nov 24]; 12(5): 610. Available from: https://www.mdpi.com/2218-273X/12/5/610 CrossRef

Plaksina EM, Sidorenko OC, Bozhok GA. Cryopreservation of multicellular spheroids derived from newborn piglet adrenal glands. Probl Cryobiol Cryomed. 2017; 27(4): 322-33. CrossRef

Seliuta AA, Polyakova AL, Gurina TM. Hyaluronic acid: innovations and prospects in biology and medicine. Regul Mech Biosyst. 2025 Sep 24 [cited 2025 Nov 24]; 16(4): e25167. Available from: https://medicine.dp.ua/index.php/med/article/view/1439 CrossRef

Ujihira M, Iwama A, Aoki M, et al. Cryoprotective effect of low-molecular-weight hyaluronan on human dermal fibroblast monolayers. CryoLetters. 2010; 31(2): 101-11. PubMed

Загрузки

Опубликован

2026-05-24

Как цитировать

Seliuta, A., Smolyaninova, Y., Kovalenko, S., Tymofieieva, O., Poliakova, H., & Gurina, T. (2026). Cryopreservation of L929 cell culture in protective solutions containing hyaluronic acid. Проблемы криобиологии и криомедицины, 36(1), 27–31. извлечено от https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/2181

Выпуск

Раздел

Теоретическая и экспериментальная криобиология