Preservation of Viability and Biological Properties of S. aureus and P. aeruginosa after 3 Years’ Storage under –196°C

Authors

  • I. P. Vysekantsev
  • S. V. Koschij
  • E. V. Kudokotseva
  • O. V. Shapovalova
  • O. N. Belokon

Keywords:

bacteria, cryopreservation, biological properties

Abstract

The storage of S. aureus and P. aeruginosa bacteria under liquid nitrogen temperature during 3 years provided the preservation for viable cells and biological properties, used for taxonomy and identification of mentioned bacteria. The death of a part of cells is possible at the stage of cooling. Meat-peptone broth (MPB) with sucrose and DMSO additives was used as the preservation medium.

Author Biographies

I. P. Vysekantsev

Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

 

S. V. Koschij

Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

 

E. V. Kudokotseva

Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

 

O. V. Shapovalova

Institute of Dermatology and Venerology of the Academy of Medical Sciences of the Ukraine, Kharkiv

 

O. N. Belokon

Institute of Dermatology and Venerology of the Academy of Medical Sciences of the Ukraine, Kharkiv

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Published

2006-06-25

How to Cite

Vysekantsev, I. P., Koschij, S. V., Kudokotseva, E. V., Shapovalova, O. V., & Belokon, O. N. (2006). Preservation of Viability and Biological Properties of S. aureus and P. aeruginosa after 3 Years’ Storage under –196°C. Problems of Cryobiology and Cryomedicine, (2), 115–120. Retrieved from https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/1037

Issue

Section

Cryopreservation of Biological Resources