Optimizing Method for Potato Meristem Vitrification

Authors

  • T. F. Stribul
  • N. A. Shevchenko
  • L. F. Rozanov

Keywords:

Solanum tuberosum, meristem, cryopreservation, vitrification, vitrification solution

Abstract

Comparative data of potato meristem cryopreservation according to different freezing protocols are shown. The possibility to vary conditions for apexes (shoot-tips) preparing to cryopreservation using vitrification method was investigated. There was proposed a vitrification PVSN solution with modified composition. A possibility to exclude some steps of preliminary meristem preparing to freezing with no loss in integrity of cryopreserved material was established.

Author Biographies

T. F. Stribul

Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

N. A. Shevchenko

Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

L. F. Rozanov

Institute for Problems of Cryobiology and Cryomedicine of the National Academy of Sciences of Ukraine, Kharkiv

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Published

2005-12-25

How to Cite

Stribul, T. F., Shevchenko, N. A., & Rozanov, L. F. (2005). Optimizing Method for Potato Meristem Vitrification. Problems of Cryobiology and Cryomedicine, 15(4), 657–664. Retrieved from https://cryo.org.ua/journal/index.php/probl-cryobiol-cryomed/article/view/1215

Issue

Vol. 15 No. 4 (2005): Problems of Cryobiology

Section

Cryopreservation of Biological Resources

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